Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa

• DOI: 10.1038/srep38795
• Publication Date: 2016-12-09T13:00:28Z
• Authors: Yuhui Yang; Shuguang Lu; Wei Shen; Xia Zhao; Mengyu Shen; Yinling Tan; Gang Li; Ming Li; Jing Wang; Fuquan Hu; Shuai Le
• Keywords: RNA Silencing; Phage therapy; Bacterial virus

Bacteriophages (phages) are widely distributed in the biosphere and play a key role modulating microbial ecology soil, ocean, humans. Although of DNA bacteriophages is well described, biology RNA poorly understood. More than 1900 phage genomes currently deposited NCBI, but only 6 dsRNA 12 ssRNA genome sequences reported. The were isolated from legume samples or lakes with Pseudomonas syringae as host. Here, we report first aeruginosa phiYY three-segmented genome. was hospital sewage China clinical P. strain, PAO38, Moreover, has broad host range, which infects 99 out 233 strains four provinces China. This work presented detailed characterization bacteriophage infecting aeruginosa.