LncRNAs expression profiling in normal ovary, benign ovarian cyst and malignant epithelial ovarian cancer

• DOI: 10.1038/srep38983
• Publication Date: 2016-12-12T10:07:24Z
• Authors: Huan Wang; Ziyi Fu; Chencheng Dai; Jian Cao; Xiaoguang Liu; Juan Xu; Mingming Lv; Yun Gu; Jingmin Zhang; Xiangdong Hua; Genmei Jia; Sujuan Xu; Xuemei Jia; Pengfei Xu
• Keywords: Adult; Ovarian Neoplasms; 0303 health sciences; Gene Expression Profiling; Carcinoma; Ovary; Carcinoma, Ovarian Epithelial; Middle Aged; Article; 3. Good health; Gene Expression Regulation, Neoplastic; Ovarian Cysts; Young Adult; 03 medical and health sciences; Humans; Female; RNA, Long Noncoding; Neoplasms, Glandular and Epithelial; RNA, Messenger; Transcriptome; Aged; Oligonucleotide Array Sequence Analysis

AbstractLong noncoding RNA (lncRNA) has been recognized as a regulator of gene expression, and the dysregulation of lncRNAs is involved in the progression of many types of cancer, including epithelial ovarian cancer (EOC). To explore the potential roles of lncRNAs in EOC, we performed lncRNA and mRNA microarray profiling in malignant EOC, benign ovarian cyst and healthy control tissues. In this study, 663 transcripts of lncRNAs were found to be differentially expressed in malignant EOC compared with benign and normal control tissues. We also selected 18 altered lncRNAs to confirm the validity of the microarray analysis using quantitative real-time PCR (qPCR). Pathway and Gene Ontology (GO) analyses demonstrated that these altered transcripts were involved in multiple biological processes, especially the cell cycle. Furthermore, Series Test of Cluster (STC) and lncRNA-mRNA co-expression network analyses were conducted to predict lncRNA expression trends and the potential target genes of lncRNAs. We also determined that two antisense lncRNAs (RP11-597D13.9 and ADAMTS9-AS1) were associated with their nearby coding genes (FAM198B, ADAMTS9), which participated in cancer progression. This study offers helpful information to understand the initiation and development mechanisms of EOC.