Development of a high-density genetic linkage map and identification of flowering time QTLs in adzuki bean (Vigna angularis)

• DOI: 10.1038/srep39523
• Publication Date: 2016-12-24T20:56:02Z
• Authors: Changyou Liu; Baojie Fan; Zhimin Cao; Qiuzhu Su; Yan Wang; Zhixiao Zhang; Jing Tian
• Keywords: Genetic linkage; Synteny; Positional cloning; Genetic linkage map; Linkage (software); Inbred strain

A high-density linkage map is crucial for the identification of quantitative trait loci (QTLs), positional cloning, and physical assembly. Here, we report development a based on specific length amplified fragment sequencing (SLAF-seq) adzuki bean flowering time-related QTLs. Through SLAF library construction Illumina recombinant inbred line (RIL) population, total 4425 markers were developed assigned to 11 groups (LGs). After binning that represented same genotype, final 1628.15 cM contained 2032 markers, with an average marker density 0.80 cM. Comparative analysis showed high collinearity two maps degree synteny reference genome common (Phaseolus vulgaris). Using this map, one major QTL LG03 minor QTLs LG05 associated first time (FLD) consistently identified in tests over two-year period. These results provide foundation will be useful future genomic research, such as identifying other important traits, comparative mapping legumes.