RNA-seq based detection of differentially expressed genes in the skeletal muscle of Duroc pigs with distinct lipid profiles

• DOI: 10.1038/srep40005
• Publication Date: 2017-02-14T10:15:49Z
• Authors: T. F. Cardoso; A. Cánovas; O. Canela-Xandri; R. González-Prendes; M. Amills; R. Quintanilla
• Keywords: Lipogenesis; Medius; Intramuscular fat; Fold change; RNA-Seq

Abstract We have used a RNA-seq approach to investigate differential expression in the skeletal muscle of swine (N = 52) with divergent lipid profiles i.e. HIGH (increased intramuscular fat and saturated monounsaturated fatty acid contents, higher serum concentrations fatness) LOW pigs (leaner an increased polyunsaturated content). The number mRNAs non-coding RNAs (ncRNAs) expressed porcine gluteus medius were 18,104 1,558, respectively. At nominal level significance ( P -value ≤ 0.05), we detected 1,430 mRNA 12 RNA (ncRNA) transcripts as differentially (DE) vs pigs. This smaller contribution ncRNAs may biological technical reasons. performed second analysis, that was more stringent 0.01 fold-change ≥ 1.5), only 96 0 mRNA-and ncRNA-encoding genes happened be DE, subset DE enriched pathways related (lipogenesis triacylglycerol degradation) glucose metabolism. Moreover, showed lipogenic profile than their counterparts.