Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma

• DOI: 10.1038/srep40286
• Publication Date: 2017-01-13T10:31:36Z
• Authors: Anders Etzerodt; Ronan M. G. Berg; Ronni R. Plovsing; Morten N. Andersen; Magali Bebien; Mohamed Habbeddine; Toby Lawrence; Holger J. Møller; Søren K. Moestrup
• Keywords: Ectodomain; CD163

Abstract CD163 is the macrophage receptor for uptake of hemoglobin-haptoglobin complexes. The human can be shed from surface owing to a cleavage site inflammation-inducible TACE/ADAM17 enzyme. Accordingly, plasma ‘soluble CD163’ (sCD163) has become biomarker activity and inflammation. present study disclosed that 10% sCD163 in healthy persons actually extracellular vesicle (EV)-associated not being cleaved shed. Endotoxin injection volunteers caused selective increase ectodomain CD163, while septic patients exhibited high levels both soluble (EV) latter representing up 60% total CD163. A poor prognosis measured as sequential organ failure assessment (SOFA) score correlated with membrane-associated Our results show EV are part separate response context systemic While released during acute inflammatory response, this case instead may later phase response. measurement two forms constituting therefore add diagnostic prognostic value.