CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

• DOI: 10.1038/srep40942
• Publication Date: 2017-01-20T11:04:06Z
• Authors: Jane C. Edwards; Helen E. Everett; Miriam Pedrera; Helen Mokhtar; Emanuele Marchi; Ferran Soldevila; Daryan A. Kaveh; Philip J. Hogarth; Helen L. Johns; Javier Nunez-Garcia; Falko Steinbach; Helen R. Crooke; Simon P. Graham
• Keywords: CD1

Abstract Conventional dendritic cells (cDC) are professional antigen-presenting that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants recently pigs. Pigs an important biomedical model species a key source of animal protein; therefore further understanding their system will help underpin the development disease prevention strategies. To characterise cDC populations porcine blood, DC were enriched from PBMC by CD14 depletion CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8α, CD21) specific for CD172a, CD1 CD4. distinct subpopulations FACSorted − (Lin CD172 + CD4 ) ), characterised phenotypic functional analyses. cDC, expressing higher levels MHC class II CD11b. Following TLR stimulation, produced IL-8 IL-10 while secreted IFN-α, IL-12 TNF-α. superior stimulating allogeneic T cell responses cross-presenting viral antigens to CD8 cells. Comparison transcriptional profiles suggested orthologues cDC2 subsets respectively.