LanCL proteins are not Involved in Lanthionine Synthesis in Mammals

• DOI: 10.1038/srep40980
• Publication Date: 2017-01-20T11:15:41Z
• Authors: Chang He; Min Zeng; Debapriya Dutta; Tong Hee Koh; Jie Chen; Wilfred A. van de...
• Keywords: Lanthionine; Lantibiotics; Dehydroalanine

LanC-like (LanCL) proteins are mammalian homologs of bacterial LanC enzymes, which catalyze the addition thiol Cys to dehydrated Ser residues during biosynthesis lanthipeptides, a class natural products formed by post-translational modification precursor peptides. The functions LanCL currently unclear. A recent proposal suggested that LanCL1 catalyzes glutathione protein- or peptide-bound dehydroalanine (Dha) form lanthionine, analogous reaction catalyzed in bacteria. Lanthionine has been detected human brain as downstream metabolite lanthionine ketimine (LK), shown have neuroprotective effects. In this study, we tested is involved constructing knock-out mice and measuring LK concentrations their brains using mass spectrometric detection method developed for purpose. To investigate whether other (LanCL2/3) may confer compensatory effect, triple (TKO) were also generated tested. Very similar (0.5-2.5 nmol/g tissue) found knock-out, TKO wild type (WT) mouse brains, suggesting not biosynthesis.