DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response

• DOI: 10.1038/srep41010
• Publication Date: 2017-02-08T11:53:24Z
• Authors: Chunmei He; Zhenming Yu; Jaime A. Teixeira...; Jianxia Zhang; Xuncheng Liu; Xiaojuan Wang; Xinhua Zhang; Songjun Zeng; Kunlin Wu; Jianwen Tan; Guohua Ma; Jianping Luo; Jun Duan
• Keywords: Dendrobium; Water Stress

GDP-mannose pyrophosphorylase (GMP) catalyzed the formation of GDP-mannose, which serves as a donor for biosynthesis mannose-containing polysaccharides. In this study, three GMP genes from Dendrobium officinale (i.e., DoGMPs) were cloned and analyzed. The putative 1000 bp upstream regulatory region these DoGMPs was isolated cis-elements identified, indicates their possible role in responses to abiotic stresses. DoGMP1 protein shown be localized cytoplasm. To further study function gene, 35S:DoGMP1 transgenic A. thaliana plants with an enhanced expression level generated. Transgenic indistinguishable wild-type (WT) tissue culture or soil. However, mannose content extracted water-soluble polysaccharides increased 67%, 96% 92% lines #1, #2 #3, respectively more than WT levels. Germination percentage seeds higher growth seedlings better under salinity stress (150 mM NaCl). Our results provide genetic evidence involvement mediation response salt during seed germination seedling growth.