Biological Membrane-Packed Mesenchymal Stem Cells Treat Acute Kidney Disease by Ameliorating Mitochondrial-Related Apoptosis

• DOI: 10.1038/srep41136
• Publication Date: 2017-01-24T10:35:09Z
• Authors: Xiaodong Geng; Quan Hong; Weiwei Wang; Wei Zheng; Ou Li; Guangyan Cai; Xiangmei Chen; Di Wu
• Keywords: Glycerol; Male; 0301 basic medicine; Cell Survival; Cell Membrane; Cell- and Tissue-Based Therapy; Apoptosis; Mesenchymal Stem Cells; Acute Kidney Injury; Cadherins; Article; Rhabdomyolysis; Cell Line; Mitochondria; 3. Good health; Mice, Inbred C57BL; 03 medical and health sciences; Animals; Humans

Abstract The mortality of rhabdomyolysis-induced AKI remains high because no effective therapy exists. We investigated a new therapeutic method using MSCs. aim this study was to investigate the potential and anti-apoptotic mechanisms action MSCs in treatment induced by glycerol vivo vitro . used Duragen as biological membrane pack on glycerol-injured renal tissue mechanism investigated. In , HK-2 cells were incubated with ferrous myoglobin MSCs-conditioned medium, followed cell proliferation apoptosis assays. founded that packing injured preserved function, ameliorated tubular lesions, reduced mice glycerol-induced AKI. MSC-conditioned medium improved viability inhibited apoptosis. These effects reversed PI3K inhibitor LY294002. Biological has rescue function inhibiting protect from have thus demonstrated rhabdomyolysis-associated injury activating PI3K/Akt pathway