Quantification of Detergents Complexed with Membrane Proteins

Models, Molecular 0301 basic medicine 570 [SDV]Life Sciences [q-bio] Detergents Aucun [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics Bioengineering Sensitivity and Specificity [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Article Analytical Chemistry 03 medical and health sciences Models 616 Matrix-Assisted Laser Desorption-Ionization Micelles 0303 health sciences Molecular Biology/Structural Biology [q-bio.BM] Spectrometry Molecular Membrane Proteins Reproducibility of Results [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Biological Sciences Mass 540 [SDV] Life Sciences [q-bio] Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Chemical Sciences Liposomes Biochemistry and Cell Biology Generic health relevance Molecular Biology/Biophysics
DOI: 10.1038/srep41751 Publication Date: 2017-02-08T11:55:39Z
ABSTRACT
Abstract Most membrane proteins studies require the use of detergents, but because lack a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional structural data analyses. To solve this problem, we propose based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) allows quantification pure or mixed detergents in complex with proteins. We validated wide variety automated process, thereby allowing routine for broad spectrum usage. As first illustration, show how obtain information amount detergent protein, essential liposome nanodiscs reconstitutions. Thanks method, also reliably easily estimate corona diameter select smallest size, critical favoring protein-protein contacts triggering/promoting protein crystallization, visualize belt Cryo-EM studies.
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