Quantification of Detergents Complexed with Membrane Proteins
Models, Molecular
0301 basic medicine
570
[SDV]Life Sciences [q-bio]
Detergents
Aucun
[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
Bioengineering
Sensitivity and Specificity
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Article
Analytical Chemistry
03 medical and health sciences
Models
616
Matrix-Assisted Laser Desorption-Ionization
Micelles
0303 health sciences
Molecular Biology/Structural Biology [q-bio.BM]
Spectrometry
Molecular
Membrane Proteins
Reproducibility of Results
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Biological Sciences
Mass
540
[SDV] Life Sciences [q-bio]
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Sciences
Liposomes
Biochemistry and Cell Biology
Generic health relevance
Molecular Biology/Biophysics
DOI:
10.1038/srep41751
Publication Date:
2017-02-08T11:55:39Z
AUTHORS (20)
ABSTRACT
Abstract Most membrane proteins studies require the use of detergents, but because lack a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional structural data analyses. To solve this problem, we propose based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) allows quantification pure or mixed detergents in complex with proteins. We validated wide variety automated process, thereby allowing routine for broad spectrum usage. As first illustration, show how obtain information amount detergent protein, essential liposome nanodiscs reconstitutions. Thanks method, also reliably easily estimate corona diameter select smallest size, critical favoring protein-protein contacts triggering/promoting protein crystallization, visualize belt Cryo-EM studies.
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CITATIONS (64)
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