Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo

• DOI: 10.1038/srep42556
• Publication Date: 2017-02-13T10:21:20Z
• Authors: Xin Qi; Peng Pei; Min Zhu; Xiaoyu Du; Chen Xin; Shichang Zhao; Xiaolin Li; Yufang Zhu
• Keywords: Bioactive Glass

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. field of tissue engineering, three-dimensional (3D) scaffolds are promising for treatment defects. this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) were successfully fabricated using 3D printing technique, which had regular uniform square macroporous structure, high porosity excellent apatite mineralization ability. Human marrow-derived mesenchymal stem cells (hBMSCs) cultured on to evaluate hBMSC attachment, proliferation osteogenesis-related gene expression. Critical-sized rat calvarial applied investigate effect CSH/MBG regeneration in vivo. The vitro results showed that stimulated adhesion, proliferation, alkaline phosphatase (ALP) activity expression hBMSCs. vivo could significantly enhance new formation compared CSH scaffolds. Thus printed would be candidates promoting regeneration.