Circulating classical monocytes are associated with CD11c+ macrophages in human visceral adipose tissue

• DOI: 10.1038/srep42665
• Publication Date: 2017-02-15T10:32:51Z
• Authors: Kristiaan Wouters; Katrien Gaens; Mitchell Bijnen; Kenneth Verboven; Johan Jocken; Suzan Wetzels; Erwin Wijnands; Dominique Hansen; Marleen van Greev...; Adriaan Duijvestijn; Erik A. L. Biessen; Ellen E. Blaak; Coen D. A. Stehouwer; Casper G. Schalkwijk
• Keywords: CD11c; CX3CR1; Monocyte; Adipose tissue macrophages

Abstract Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting metabolic dysfunction. Circulating immune patterns may reflect expanding AT. However, data linking human leukocytes blood and lacking. We investigated whether populations are their counterparts subcutaneous (scAT) or visceral (vAT). Flow cytometry was performed on blood, scAT vAT from 16 lean 29 obese men. natural killer (NK)-cells, classical monocytes nonclassical were higher individuals. vAT, but not scAT, individuals contained more inflammatory CD11c + “M1” macrophages NK cells compared to Blood scAT. This association unrelated expression adhesion molecules CD11b chemokine receptor CX3CR1 these monocytes. Other respective blood. Finally, CD4 T-cells In conclusion, vAT.