Building a 5-HT3A Receptor Expression Map in the Mouse Brain

• DOI: 10.1038/srep42884
• Publication Date: 2017-03-09T11:32:48Z
• Authors: Yoshihisa Koyama; Makoto Kondo; Shoichi Shimada
• Keywords: Male; 0301 basic medicine; Brain; Mice, Transgenic; Synaptic Transmission; Article; 3. Good health; Mice; 03 medical and health sciences; Interneurons; Animals; Tissue Distribution; GABAergic Neurons; Receptors, Serotonin, 5-HT3

Abstract Of the many serotonin receptors, type 3 receptors (5-HT3R) are only ionotropic ones, playing a key role in fast synaptic transmission and cognitive emotional brain function through controlled neuronal excitation. To better understand various functions of 5-HT3Rs, it is very important to know their expression pattern central nervous system (CNS). date, distributional studies have shown localized 5-HT3R spinal cord. However, an accurate CNS remains be elucidated. investigate distribution mouse detail, we performed immunofluorescent staining using 5-HT3AR-GFP transgenic mice. We found strong 5-HT3AR olfactory bulb, cerebral cortex, hippocampus, amygdala; partial pons, medulla, Meanwhile, thalamus, hypothalamus, midbrain exhibited few 5-HT3AR-expressing cells, no was detected cerebellum. Further, double-immunostaining neural markers confirmed that expressed GABAergic interneurons containing somatostatin or calretinin. In present study, built map brain. Our findings make significant contributions elucidating novel CNS.