Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling

• DOI: 10.1038/srep43134
• Publication Date: 2017-02-24T10:49:17Z
• Authors: Hiroaki Ito; Ryo Murakami; Shinya Sakuma; Chia-Hung Dylan Tsai; Thomas Gutsmann; Klaus Brandenburg; Johannes M. B. Pö...; Fumihito Arai; Makoto Kaneko; Motomu Tanaka
• Keywords: Adenosine triphosphate; Erythrocyte deformability

Abstract Large deformability of erythrocytes in microvasculature is a prerequisite to realize smooth circulation. We develop novel tool for the three-step “Catch-Load-Launch” manipulation human erythrocyte based on an ultra-high speed position control by microfluidic “robotic pump”. Quantification shape recovery as function loading time uncovered critical window transition between fast and slow recoveries. The comparison with under depletion adenosine triphosphate revealed that cytoskeletal remodeling over whole cell occurs 3 orders magnitude longer timescale than local dissociation-reassociation single spectrin node. Finally, we modeled septic conditions incubating endotoxin, found exposure endotoxin results significant delay characteristic remodeling. high robotic pump technique allows throughput mechanical diagnosis blood-related diseases.