Identification of candidate diagnostic serum biomarkers for Kawasaki disease using proteomic analysis

• DOI: 10.1038/srep43732
• Publication Date: 2017-03-06T11:04:48Z
• Authors: Yayoi Kimura; Masakatsu Yanagim...; Yoko Ino; Mao Aketagawa; Michie Matsuo; Akiko Okayama; Hiroyuki Shimizu; Kunihiro Oba; Ichiro Morioka; Tomoyuki Imagawa; Tetsuji Kaneko; Shumpei Yokota; Hisashi Hirano; Masaaki Mori
• Keywords: Retinol binding protein; Systemic vasculitis

Abstract Kawasaki disease (KD) is a systemic vasculitis and childhood febrile that can lead to cardiovascular complications. The diagnosis of KD depends on its clinical features, thus it sometimes difficult make definitive diagnosis. In order identify diagnostic serum biomarkers for KD, we explored KD-related proteins, which differentially expressed during the acute recovery phases two patients by mass spectrometry (MS). We identified total 1,879 proteins MS-based proteomic analysis. levels three these namely lipopolysaccharide-binding protein (LBP), leucine-rich alpha-2-glycoprotein (LRG1), angiotensinogen (AGT), were higher in phase patients. contrast, level retinol-binding 4 (RBP4) was decreased. To confirm usefulness as biomarkers, analyzed 270 samples, including those collected from 55 with using western blot analysis microarray enzyme-linked immunosorbent assays (ELISAs). Over course this experiment, determined expression changes specifically rather than or other illness. Thus, LRG1 could be used facilitate based features.