High glucose alters tendon homeostasis through downregulation of the AMPK/Egr1 pathway

• DOI: 10.1038/srep44199
• Publication Date: 2017-03-07T10:53:43Z
• Authors: Yu-Fu Wu; Hsing-Kuo Wang; Hong-Wei Chang; Jingyu Sun; Jui-Sheng Sun; Yuan-Hung Chao
• Keywords: EGR1

Abstract Diabetes mellitus (DM) is associated with higher risk of tendinopathy, which reduces tolerance to exercise and functional activities affects lifestyle glycemic control. Expression tendon-related genes matrix metabolism in tenocytes are essential for maintaining physiological functions tendon. However, the molecular mechanisms involved diabetic tendinopathy remain unclear. We hypothesized that high glucose (HG) alters characteristics tenocyte. Using vitro 2-week culture tenocytes, we found expression genes, including Egr1, Mkx, TGF-β1, Col1a2, Bgn, was significantly decreased HG consumption occurred. Down-regulation Egr1 by siRNA Scx, Col1a1, Bgn expression. Blocking AMPK activation Compound C reduced low condition. In addition, histological examination tendons from mice displayed larger interfibrillar space uneven glycoprotein deposition. Thus, concluded tendon homeostasis through downregulation AMPK/Egr1 pathway downstream tenocytes. The findings render a basis mechanism may help develop preventive therapeutic strategies pathology.