Characterization of the polyphenol oxidase gene family reveals a novel microRNA involved in posttranscriptional regulation of PPOs in Salvia miltiorrhiza

• DOI: 10.1038/srep44622
• Publication Date: 2017-03-17T12:37:58Z
• Authors: Caili Li; Dongqiao Li; Jiang Li; Fenjuan Shao; Shanfa Lu
• Keywords: Salvia miltiorrhiza

Abstract Salvia miltiorrhiza is a well-known material of traditional Chinese medicine. Understanding the regulatory mechanisms phenolic acid biosynthesis and metabolism are important for S. quality improvement. We report here that contains 19 polyphenol oxidases ( PPOs ), forming largest PPO gene family in plant species to our knowledge. Analysis structures sequence features revealed conservation divergence SmPPOs. SmPPOs were differentially expressed tissues eight them predominantly phloem xylem, indicating some functionally redundant, whereas others associated with different physiological processes. Expression patterns eighteen significantly altered under MeJA treatment, twelve yeast extract Ag + -responsive, suggesting majority stress-responsive. high-throughput small RNA sequences degradome data showed miR1444-mediated regulation existing P. trichocarpa absent from . Instead, subset was posttranscriptionally regulated by novel miRNA, termed Smi-miR12112. It indicates specificity significance miRNA-mediated The results shed light on SmPPO expression suggest complexity SmPPO-associated metabolism.