Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery

• DOI: 10.1038/srep44910
• Publication Date: 2017-03-21T14:35:18Z
• Authors: Hongyu Zhang; Fangyan Chen; Xu Fan; Cong Lin; Yunwei Hao; Handong Wei; Weiran Lin; Ying Jiang; Fuchu He
• Keywords: Reversion; Hepatic stellate cell; Proteome

Abstract Understanding the changes of activated HSCs reversion is an essential step toward clarifying potential roles in treatment liver fibrosis. In this study, we chose adipocyte differentiation mixture to induce LX-2 cells for 2 days vitro as phase, comparing with normal cultured activation phase. Mass spectrometric-based SILAC technology was adopted study differentially expressed proteome between and activation. Compared HSCs, 273 proteins showed significant differences reverted HSCs. The main pathway up-regulated associated mainly related oxidation-reduction lipid metabolism, while top down-regulated found regulated cytoskeleton formation. Changes expression levels selected were verified by Western blotting analysis, especially STAT1, FLNA, LASP1, NAMPT proteins. distinct STAT1 further analyzed it that could affect cell proliferation be viewed a key regulator Thus, proteomic analysis accelerate our understanding mechanisms HSC on cessation fibrogenic stimuli provide new targets antifibrotic therapy.