Genomic characterization of Nontuberculous Mycobacteria

• DOI: 10.1038/srep45258
• Publication Date: 2017-03-27T09:47:58Z
• Authors: Tarcisio Fedrizzi; Conor J. Meehan; Antonella Grottola; Elisabetta Giacob...; Giulia Fregni Ser...; Sara Tagliazucchi; Anna Fabio; Clotilde Bettua; Roberto Bertorelli; Veronica De Sanctis; Fabio Rumpianesi; Monica Pecorari; Olivier Jousson; Enrico Tortoli; Nicola Segata
• Keywords: 0301 basic medicine; Chromosome Mapping; Gene Transfer, Horizontal; Genetic Variation; Genome, Bacterial; Humans; Molecular Sequence Annotation; Mycobacterium Infections, Nontuberculous; Nontuberculous Mycobacteria; Open Reading Frames; Phylogeny; Sequence Analysis, DNA; Multidisciplinary; 0303 health sciences; Genome; Gene Transfer, Horizontal; Chromosome Mapping; Genetic Variation; Mycobacterium Infections, Nontuberculous; Molecular Sequence Annotation; Nontuberculous Mycobacteria; Sequence Analysis, DNA; Article; 3. Good health; Open Reading Frames; 03 medical and health sciences; Humans; Genome, Bacterial; Phylogeny

AbstractMycobacterium tuberculosis and Mycobacterium leprae have remained, for many years, the primary species of the genus Mycobacterium of clinical and microbiological interest. The other members of the genus, referred to as nontuberculous mycobacteria (NTM), have long been underinvestigated. In the last decades, however, the number of reports linking various NTM species with human diseases has steadily increased and treatment difficulties have emerged. Despite the availability of whole genome sequencing technologies, limited effort has been devoted to the genetic characterization of NTM species. As a consequence, the taxonomic and phylogenetic structure of the genus remains unsettled and genomic information is lacking to support the identification of these organisms in a clinical setting. In this work, we widen the knowledge of NTMs by reconstructing and analyzing the genomes of 41 previously uncharacterized NTM species. We provide the first comprehensive characterization of the genomic diversity of NTMs and open new venues for the clinical identification of opportunistic pathogens from this genus.