Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco

• DOI: 10.1038/srep45331
• Publication Date: 2017-03-27T09:43:42Z
• Authors: Diego Hidalgo; Ascensión Martíne...; Elisabeth Moyano; Roque Bru-Martínez; Purificación Corc...; Javier Palazon
• Keywords: Pterostilbene; Bioconversion; Piceatannol; Biotransformation; Heterologous expression

It is currently possible to transfer a biosynthetic pathway from plant another organism. This system has been exploited the metabolic richness of certain species other plants or even more simple organisms such as yeast bacteria for production high added value compounds. Another application bioconvert substrates into scarcer biologically interesting compounds, piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are found in only small amounts. By transferring human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) gene tobacco hairy roots cell cultures, we developed able exogenous t-resveratrol quantities near mg L-1. Similarly, after heterologous expression resveratrol O-methyltransferase Vitis vinifera (VvROMT) roots, was bioconverted We also observed that both bioconversions can take place wild type (pRiA4, without any transgene), showing unspecific hydroxylases methyltransferases perform bioconversion give target albeit at lower rate than transgenic roots.