Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco
Bioconversion
Nicotiana
0301 basic medicine
Plant genetics
Genetically engineered root
Cell Culture Techniques
Molecular engineering in plants
Plant Roots
7. Clean energy
Article
Mixed Function Oxygenases
12. Responsible consumption
03 medical and health sciences
Gene Expression Regulation, Plant
Tobacco
Stilbenes
Humans
Vitis
Plant Proteins
0303 health sciences
Genètica vegetal
Methyltransferases
Bioquímica y Biología Molecular
Cell cultures
Plants, Genetically Modified
Biosynthetic Pathways
Resveratrol
Cytochrome P-450 CYP1B1
Genetic Engineering
Metabolic engineering
DOI:
10.1038/srep45331
Publication Date:
2017-03-27T09:43:42Z
AUTHORS (6)
ABSTRACT
AbstractIt is currently possible to transfer a biosynthetic pathway from a plant to another organism. This system has been exploited to transfer the metabolic richness of certain plant species to other plants or even to more simple metabolic organisms such as yeast or bacteria for the production of high added value plant compounds. Another application is to bioconvert substrates into scarcer or biologically more interesting compounds, such as piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are found in plants only in small amounts. By transferring the human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) gene to tobacco hairy roots and cell cultures, we developed a system able to bioconvert exogenous t-resveratrol into piceatannol in quantities near to mg L−1. Similarly, after heterologous expression of resveratrol O-methyltransferase from Vitis vinifera (VvROMT) in tobacco hairy roots, the exogenous t-resveratrol was bioconverted into pterostilbene. We also observed that both bioconversions can take place in tobacco wild type hairy roots (pRiA4, without any transgene), showing that unspecific tobacco P450 hydroxylases and methyltransferases can perform the bioconversion of t-resveratrol to give the target compounds, albeit at a lower rate than transgenic roots.
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