Preparation and characterization of (−)-Epigallocatechin-3-gallate (EGCG)-loaded nanoparticles and their inhibitory effects on Human breast cancer MCF-7 cells

• DOI: 10.1038/srep45521
• Publication Date: 2017-03-28T09:12:01Z
• Authors: Liang Zeng; Jingna Yan; Liyong Luo; Mengjun Ma; Huiqun Zhu
• Keywords: MCF-7; IC50; Gallate

We were employing nanotechnology to improve the targeting ability of (-)-Epigallocatechin-3-gallate (EGCG) towards MCF-7 cells, and two kinds EGCG nanoparticles (FA-NPS-PEG FA-PEG-NPS) obtained, besides, their characteristics effects on cells studied. The results indicated that (i) both FA-NPS-PEG FA-PEG-NPS have high stabilities; (ii) particles sizes 185.0 ± 13.5 nm 142.7 7.2 nm, respectively; (iii) encapsulation efficiencies 90.36 2.20% 39.79 7.54%, respectively. (iv) there was no cytotoxicity observed in EGCG, toward over all concentrations (0~400 μg/mL) tested; (v) inhibited proliferation dose-dependent manners, with average IC50 470.5 33.0, 65.9 0.4 66.6 0.6 μg/mL; (vi) could modulated expressions several key regulatory proteins PI3K-Akt pathway such as up-regulation PTEN, p21 Bax, down-regulation p-PDK1, p-AKT, CyclinD1 Bcl-2, which gave an illustration about mechanism by proliferation. In this study, can significantly enhance efficacy is considered experimental foundation for further research its activity, metabolism vivo.