Ionizing radiation abrogates the pro-tumorigenic capacity of cancer-associated fibroblasts co-implanted in xenografts

• DOI: 10.1038/srep46714
• Publication Date: 2017-04-25T09:45:34Z
• Authors: Maria Tunset Grinde; Jørg Vik; Ketil André Camilio; Inigo Martinez-Zu...; Turid Hellevik
• Keywords: Cancer-Associated Fibroblasts

Abstract Cancer-associated fibroblasts (CAFs) are abundantly present in solid tumors and affect tumorigenesis therapeutic responses. In the context of clinical radiotherapy, impact irradiated CAFs to treatment outcomes is largely unexplored. Aiming at improving radiotherapy efficacy, we have here explored effect radiation on inherent pro-tumorigenic capacity animals. Ionizing was delivered cultured as single-high or fractionated doses. Tumor development compared mice receiving A549 lung tumor cells admixed with control CAFs. Biological mechanisms behind growth regulation were investigated by quantitative histology immunohistochemistry. Viability assessments confirmed that fully functional prior implantation. However, enhanced tumorigenic observed co-implanted abrogated established Experiments ascertain fate implanted showed exogenously administered reside implantation site for few days, suggesting from take place during initial formation. Our work demonstrate lose their potential vivo , affecting angiogenesis engraftment. This finding propose a previously unknown advantageous induced adding direct cytotoxic effects transformed epithelial cells.