Enhanced sensitivity for the determination of endogenous phylloquinone (vitamin K1) in plasma using high-performance liquid chromatography with dual-electrode electrochemical detection
0404 agricultural biotechnology
Electrochemistry
Humans
Vitamin K 1
04 agricultural and veterinary sciences
Oxidation-Reduction
01 natural sciences
Chromatography, High Pressure Liquid
0104 chemical sciences
DOI:
10.1039/an9851001181
Publication Date:
2004-03-23T17:19:45Z
AUTHORS (3)
ABSTRACT
High-performance liquid chromatography (HPLC) with dual-electrode electrochemical detection has been used, in the redox mode, to determine normal and sub-normal circulating plasma levels of phylloquinone (vitamin K1) and concentrations down to 20 pg ml–1 have been measured in plasma. The coefficient of variation, for endogenous levels, was 10%(mean 330 pg ml–1; n= 6). When compared with a single glassy carbon electrode, operated in the reductive mode, the sensitivity of the dual-electrode cell was greater by at least an order of magnitude. Thus the volume of plasma required for the assay could be reduced.
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