Enhanced sensitivity for the determination of endogenous phylloquinone (vitamin K1) in plasma using high-performance liquid chromatography with dual-electrode electrochemical detection

Glassy carbon
DOI: 10.1039/an9851001181 Publication Date: 2004-03-23T17:19:45Z
ABSTRACT
High-performance liquid chromatography (HPLC) with dual-electrode electrochemical detection has been used, in the redox mode, to determine normal and sub-normal circulating plasma levels of phylloquinone (vitamin K1) concentrations down 20 pg ml–1 have measured plasma. The coefficient variation, for endogenous levels, was 10%(mean 330 ml–1; n= 6). When compared a single glassy carbon electrode, operated reductive sensitivity cell greater by at least an order magnitude. Thus volume required assay could be reduced.
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