Enhanced sensitivity for the determination of endogenous phylloquinone (vitamin K1) in plasma using high-performance liquid chromatography with dual-electrode electrochemical detection

• DOI: 10.1039/an9851001181
• Publication Date: 2004-03-23T17:19:45Z
• Authors: John P. Hart; Martin J. Shearer; Patrick T. McCarthy
• Keywords: Glassy carbon

High-performance liquid chromatography (HPLC) with dual-electrode electrochemical detection has been used, in the redox mode, to determine normal and sub-normal circulating plasma levels of phylloquinone (vitamin K1) concentrations down 20 pg ml–1 have measured plasma. The coefficient variation, for endogenous levels, was 10%(mean 330 ml–1; n= 6). When compared a single glassy carbon electrode, operated reductive sensitivity cell greater by at least an order magnitude. Thus volume required assay could be reduced.