Design of histidine-Zn2+ binding sites within a β-hairpin peptide: enhancement of β-sheet stability through metal complexation

• DOI: 10.1039/b100702p
• Publication Date: 2002-07-26T09:14:06Z
• Authors: Geoffrey Platt; Mark S. Searle; Chun-Wa Chung
• Keywords: Metalloprotein; Beta sheet

We describe the design and characterisation of two simple 'metalloproteins' based on Zn2+ co-ordination sites involving histidine residues close to N- C-termini β-hairpin peptides (His2-β, KHYTVSINGKKITVHI His3-β, HKHYTV-SINGKKITVHI); we show by NMR circular dichroism spectroscopy that complexation co-operatively enhances stability these partially pre-organised β-sheet peptides.