On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

0209 industrial biotechnology Hot Temperature Lasers Cell Culture Techniques Cell Separation Equipment Design 02 engineering and technology Cells, Immobilized Microfluidic Analytical Techniques Flow Cytometry 16. Peace & justice Hydrogel, Polyethylene Glycol Dimethacrylate Equipment Failure Analysis Micromanipulation Yeasts Feasibility Studies
DOI: 10.1039/b502546j Publication Date: 2005-10-17T15:09:04Z
ABSTRACT
A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation and locally thermosensitive hydrogelation is proposed in this paper. We employed a single laser beam as optical tweezers for separating a target cell and locating it adjacent to a fabricated, transparent micro heater. Simultaneously, the target cell is immobilized or partially entrapped by heating the thermosensitive hydrogel with the micro heater. The state of the thermosensitive hydrogel can be switched from sol to gel and gel to sol by controlling the temperature through heating and cooling by the micro heater. After other unwanted cells are removed by the high-speed cleaning flow in the microchannel, the entrapped cell is successfully isolated. It is possible to collect the immobilized target cell for analysis or culture by switching off the micro heater and releasing the cell from the entrapment. We demonstrated that the proposed approach is feasible for rapid manipulation, immobilization, cleaning, isolation and extraction of a single cell. The experimental results are shown here.
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