On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

• DOI: 10.1039/b502546j
• Publication Date: 2005-10-17T15:09:04Z
• Authors: Fumihito Arai; Chinaik Ng; Hisataka Maruyama; Akihiko Ichikawa; Haitham El-Shimy; Toshio Fukuda
• Keywords: Microchannel; Tweezers

A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation locally thermosensitive hydrogelation is proposed in this paper. We employed beam as optical tweezers separating target locating it adjacent to fabricated, transparent micro heater. Simultaneously, the immobilized or partially entrapped heating hydrogel with The state can be switched from sol gel controlling temperature through cooling After other unwanted cells are removed high-speed cleaning flow microchannel, successfully isolated. It possible collect analysis culture switching off heater releasing entrapment. demonstrated that feasible manipulation, immobilization, cleaning, isolation extraction cell. experimental results shown here.