A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects

• DOI: 10.1039/b612966h
• Publication Date: 2006-11-24T09:52:37Z
• Authors: Xuanhong Cheng; Daniel Irimia; Meredith Dixon; Kazuhiko Sekine; Utkan Demirci; Lee Zamir; Ronald G. Tompkins; William Rodriguez; Mehmet Toner
• Keywords: Cell counting; Point of care

Practical HIV diagnostics are urgently needed in resource-limited settings. While infection can be diagnosed using simple, rapid, lateral flow immunoassays, disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4(+) T lymphocyte. To address limitations current expensive, technically demanding and/or time-consuming approaches, we have developed simple CD4 microfluidic device. This device uses affinity chromatography operated under differential shear to specifically isolate lymphocytes with high efficiency directly from 10 microliters unprocessed, unlabeled whole blood. counts obtained an optical microscope label-free fashion. determined our matched measurements by conventional cytometry among HIV-positive subjects over wide range absolute (R(2) = 0.93). microdevice used for rapid affordable point-of-care