Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions

• DOI: 10.1039/b804986f
• Publication Date: 2008-09-12T15:16:40Z
• Authors: Kuo-Liang Liu; Chien-Chen Wu; Ying-Jung Huang; Hwei-Ling Peng; Hwan-You Chang; Pin Chang; Long Hsu; Tri-Rung Yew
• Keywords: Bacteria; Cell Survival; Fungi; Water; 02 engineering and technology; 3. Good health; Klebsiella pneumoniae; Microscopy, Electron, Transmission; Microchip Analytical Procedures; Feasibility Studies; Microtechnology; Nanoparticles; Tellurium; 0210 nano-technology

A novel and disposable microchip (K-kit) with SiO2 nano-membranes was developed used as a specimen kit for in situ imaging of living organisms an aqueous condition using transmission electron microscopy (TEM) without equipment modification. This K-kit enabled the successful TEM observation Escherichia colicells tellurite reduction process Klebsiella pneumoniae. The K. pneumoniae Saccharomyces cerevisiae can stay alive after continuous up to 14 s 42 s, respectively. Besides, different profiles cells grown aerobic anaerobic environments be clearly revealed. These results demonstrate that this paper useful observing monitoring biological processesin situ.