Integrated SPPS on continuous-flow radial microfluidic chip

• DOI: 10.1039/c0lc00542h
• Publication Date: 2011-01-26T13:30:22Z
• Authors: Weizhi Wang; Yanyan Huang; Jizhong Liu; Yunfeng Xie; Rui Zhao; Shaoxiang Xiong; Guoquan Liu; Yi Chen; Huimin Ma
• Keywords: Fluidics; Polystyrene; Lab-on-a-Chip; Cartridge; Solid-Phase Synthesis

A novel integrated continuous-flow microfluidic system was designed and fabricated for solid phase peptide synthesis (SPPS) using conventional reactants. The composed of a glass-based radial reaction chip, diffluent amino acid feeding reservoirs reagent pathways. tri-row cofferdam-fence structure supports trapping. Highly cross-linked, porous high-loading 4-(hydroxymethyl)phenoxymethyl polystyrene (HMP) beads were prepared SPPS. transfer losses, hazardous handling time-consuming processes in traditional cleavage steps avoided by being replaced with the on-chip treatment. Six peptides from an antibody affinity library against β-endorphin different lengths sequences obtained simultaneously on constructed within short time. This is automatic, integrated, effective, low-cost, recyclable environment-friendly not only SPPS but also other chemical syntheses.