Nano-structured nickel oxide based DNA biosensor for detection of visceral leishmaniasis (Kala-azar)

• DOI: 10.1039/c1an15031f
• Publication Date: 2011-05-25T10:32:36Z
• Authors: Swati Mohan; Pankaj Srivastava; S. N. Maheshwari; Shyam Sundar; Rajiv Prakash
• Keywords: Nickel oxide

Sol–gel synthesized nickel oxide (NiO) film deposited onto indium tin (ITO) coated glass plate has been utilized for the development of sensitive and stable DNA biosensor demonstrated diagnosis visceral leishmaniasis also known as Kala-azar. Leishmania specific sensor is developed by immobilizing 23mer sequence (oligonucleotide) identified from 18S rRNA gene sequences donovani. Characterization studies like X-Ray Diffraction Scanning Electron Microscopy revealed formation nano-structured NiO, while immobilization single strand (ss)-DNA was supported UV-visible, Fourier Transform Infrared Spectroscopy techniques. Response ss-DNA/NiO/ITO bioelectrode are carried out using differential pulsed voltammetry in presence methylene blue redox dye a mediator. A linear response obtained wide concentration range 2 pg ml−1 to μg complementary target genomic (disease DNA) within variation 10% 5 sets studies. The observed results hold promise not only Kala-azar patients but enormous potential nano-NiO based probe biosensors.