A novel colorimetric determination of reduced glutathione in A549 cells based on Fe3O4 magnetic nanoparticles as peroxidase mimetics

• DOI: 10.1039/c1an15718c
• Publication Date: 2011-11-23T11:30:50Z
• Authors: Yanhua Ma; Zhenyang Zhang; Cuiling Ren; Guoyun Liu; Xingguo Chen
• Keywords: Lung Neoplasms; Metal Nanoparticles; Hydrogen Peroxide; Adenocarcinoma; Glutathione; 01 natural sciences; Catalysis; Ferrosoferric Oxide; 0104 chemical sciences; Magnetics; Biomimetic Materials; Hematinics; Tumor Cells, Cultured; Humans; Colorimetry; Coloring Agents; Oxidation-Reduction; Peroxidase

In this paper, a novel and simple colorimetric method for the determination of reduced glutathione (GSH) based on Fe(3)O(4) magnetic nanoparticles (MNPs) as peroxidase mimetics was developed. The Fe(3)O(4) MNPs prepared via a coprecipitation method, which possess intrinsic peroxidase-like activity, were used as a catalyst in the color development reaction of a peroxidase substrate 2,2'-azino-bis(3-thylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and H(2)O(2). The existence of GSH can consume H(2)O(2) and cause a color change of the reaction system which can be detected by the naked eye. Accordingly, the GSH can be detected by measuring the wastage of H(2)O(2). A good linear relationship was obtained from 3.0 to 30.0 μM for GSH. Good recoveries ranging from 96.7 to 107% were obtained. Furthermore, it was used to detect GSH in A549 cells.