Histidinylated linear PEI: a new efficient non-toxic polymer for gene transfer

• DOI: 10.1039/c1cc15716g
• Publication Date: 2011-10-24T08:31:16Z
• Authors: Emilie Bertrand; Cristine Gonçalves; Ludivine Billiet; Jean Pierre Gomez; Chantal Pichon; Hervé Cheradame; Patrick Midoux; Philippe Guégan
• Keywords: 0301 basic medicine; Drug Carriers; MESH: Humans; MESH: Transfection; [SDV.BBM]Life Sciences [q-bio]/Biochemistry; 610; MESH: Polyethyleneimine; 540; Transfection; MESH: Drug Carriers; MESH: Histidine; 03 medical and health sciences; HEK293 Cells; MESH: HEK293 Cells; MESH: HeLa Cells; [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology; Humans; Polyethyleneimine; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; Histidine; Molecular Biology; HeLa Cells

A series of linear polyethylenimine (lPEI) substituted with histidine residue (His-lPEI) was synthesized using the Michael reaction in order to provide new highly efficient vectors for gene therapy applications (up to 95% of transfected cells) with remarkable low cytotoxicity compared to lPEI-based polyplexes.