A robust, high-sensitivity stealth probe for peptidases

• DOI: 10.1039/c2cc32227g
• Publication Date: 2012-05-02T10:00:58Z
• Authors: Oliver Thorn-Seshold; Monica Vargas-San...; Sean McKeon; Jens Hasserodt
• Keywords: [SDV.BIO]Life Sciences [q-bio]/Biotechnology; Molecular Structure; [CHIM.ORGA]Chemical Sciences/Organic chemistry; [CHIM]Chemical Sciences; Fluorescein; [CHIM.THER]Chemical Sciences/Medicinal Chemistry; 01 natural sciences; Fluorescent Dyes; Peptide Hydrolases; 0104 chemical sciences

A robust, modular fluorogenic probe system has been developed which allows the highly sensitive off-ON detection of aminopeptidase activity by releasing an exceptionally photostable, insoluble, phenolic ESIPT fluorophore. The probes generate no false positive signal in over 24 hours, but when activated give a within 10 minutes.