Single-cell recording and stimulation with a 16k micro-nail electrode array integrated on a 0.18 μm CMOS chip

• DOI: 10.1039/c2lc21037a
• Publication Date: 2012-02-15T15:00:25Z
• Authors: Roeland Huys; Dries Braeken; Danny Jans; Andim Stassen; Nadine Collaert; Jan Wouters; Josine Loo; Simone Severi; Frank Vleugels; Geert Callewaert; Kris Verstreken; Carmen Bartic; Wolfgang Eberle
• Keywords: Electrode array

To cope with the growing needs in research towards understanding of cellular function and network dynamics, advanced micro-electrode arrays (MEAs) based on integrated complementary metal oxide semiconductor (CMOS) circuits have been increasingly reported. Although such contain a large number sensors for recording and/or stimulation, size electrodes these chips are often larger than typical mammalian cell. Therefore, true single-cell stimulation remains challenging. Single-cell resolution can be obtained by decreasing electrodes, which inherently increases characteristic impedance noise. Here, we present an array 16 384 active monolithically chip, realized 0.18 μm CMOS technology individual cells. Successful electrical activity cardiac cells validated intracellular whole-cell patch clamp recordings presented, illustrating readout capability. Further, applying single-electrode protocol, could pace cells, demonstrating addressability. This novel electrode help pave way solving complex interactions networks.