Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

• DOI: 10.1039/c2lc21170j
• Publication Date: 2012-02-15T09:37:47Z
• Authors: Robert Burger; Patrick Reith; Gregor Kijanka; Victor Akujobi; Patrick Abgrall; Jens Ducrée
• Keywords: Trap (plumbing); Sedimentation; Particle (ecology); Bead

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles applications in bead-based assays. The uses an array geometrical V-cup barriers to trap using stopped-flow sedimentation under reproducible hydrodynamic conditions. impact parameters governing occupancy distribution capture efficiency arrayed traps are investigated. unique, nearly 100% paired with capability establish sharply peaked, single distributions enables novel, digital readout mode color-multiplexed, particle-based assays low-complexity instrumentation. presented technology marks essential step towards versatile integration bead- cell-based biological