Self-priming compartmentalization digital LAMP for point-of-care

• DOI: 10.1039/c2lc40774d
• Publication Date: 2012-08-21T10:33:16Z
• Authors: Qiangyuan Zhu; Yibo Gao; Bingwen Yu; Hao Ren; Lin Qiu; Sihai Han; Wei Jin; Qinhan Jin; Ying Mu
• Keywords: Compartmentalization (fire protection)

Digital nucleic acid amplification provides unprecedented opportunities for absolute quantification by counting of single molecules. This technique is useful molecular genetic analysis in cancer, stem cell, bacterial, non-invasive prenatal diagnosis which many biologists are interested. paper describes a self-priming compartmentalization (SPC) microfluidic chip platform performing digital loop-mediated (LAMP). The energy the pumping pre-stored degassed bulk PDMS exploiting high gas solubility PDMS; therefore, no additional structures other than channels and reservoirs required. sample oil sequentially sucked into channels, pressure difference dissolved allows self-compartmentalization without need further manipulation such as with pneumatic microvalves control systems, so on. SPC LAMP can be used like 384-well plate, so, world-to-chip fluidic interconnections avoided. contains 4 separate panels, each panel 1200 independent 6 nL chambers to detect samples simultaneously. on was tested quantitatively using β-actin DNA from humans. behavior roughly predictable two-dimensional model. uniformity analyzed fluorescent intensity fraction volume. results showed that feasibility flexibility amplifying molecules different made diluting distributing solutions. has potential meet requirements general laboratory: power-free, valve-free, operating at isothermal temperature, inexpensive, sensitive, economizing labour time reagents. disposable analytical devices appropriate air-tight packaging should point-of-care, enabling it become one common tools biology research, especially, point-of-care testing.