Sensitive and high resolution subcutaneous fluorescence in vivo imaging using upconversion nanoparticles and microarrays

• DOI: 10.1039/c3an00441d
• Publication Date: 2013-05-01T21:39:42Z
• Authors: Xin Li; Zhuoqi Li; Wupeng Gan; Tongzhou Wang; Songmin Zhao; Ying Lu; Jing Cheng; Guoliang Huang
• Keywords: Mice; Mice, Inbred BALB C; Spectrometry, Fluorescence; Subcutaneous Tissue; Tissue Array Analysis; Animals; Nanoparticles; 02 engineering and technology; 0210 nano-technology; Molecular Imaging; Skin

A sensitive and high resolution small animal in vivo imaging system using upconversion nanoparticles (UNPs) microarrays was developed. The fluorescence tomography UNPs could achieve higher precision than that ordinary fluorophores, which theoretically explained by the finite element method (FEM). Given autofluorescence-insensitive property of UNPs, a subcutaneous detection sensitivity 0.93 × 10−4 wt% be achieved with UNP volume ∼10 μL tissue phantoms. Furthermore, fluorophore (25, 50 100 μm arrays) embedded under mouse skin were prepared for detection. An optical clearing applied to enhance transparency improve spatial resolution. results demonstrated optimized microarrays. Taken together, we conclude proposed sensitive, detection, have great potential throughput tumors other diseases.