Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis

• DOI: 10.1039/c3an00830d
• Publication Date: 2013-06-10T15:05:44Z
• Authors: Juanni Zhang; Jianniao Tian; Yanlong He; Sheng Chen; Yixuan Jiang; Yanchun Zhao; Shulin Zhao
• Keywords: Aptamer; Linear range

We report a fluorescence polarization platform for H1N1 detection based on the construction of DNA functional QD probe and bi-functional protein binding aptamer (Apt-DNA). The assay has linear range from 10 nM to 100 with limit 3.45 is selective over mismatched bases.