Human plasma stability during handling and storage: impact on NMR metabolomics

• DOI: 10.1039/c3an02188b
• Publication Date: 2014-01-07T13:30:19Z
• Authors: Joana Pinto; M. Rosário M. Dom...; Eulália Galhano; Cristina Pita; Maria do Céu Almeida; Isabel M. Carreira; Ana M. Gil
• Keywords: ANTICOAGULANT; Adult; 0301 basic medicine; SPECTROSCOPY; Protein Stability; Middle Aged; HUMAN BLOOD; H-1-NMR; VALIDATION; SERUM; Specimen Handling; Young Adult; 03 medical and health sciences; Blood Preservation; SPECTRA; URINE; Humans; Metabolomics; Female; COLLECTION; Nuclear Magnetic Resonance, Biomolecular

The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions up to 5 consecutive freeze–thaw cycles.