Quantification of ovarian cancer markers with integrated microfluidic concentration gradient and imaging nanohole surface plasmon resonance

• DOI: 10.1039/c3an36616b
• Publication Date: 2013-01-10T12:31:48Z
• Authors: Carlos Escobedo; Yu-Wei Chou; Mohammad Rahman; Xiaobo Duan; Reuven Gordon; David Sinton; Alexandre G. Brolo; Jacqueline Ferreira
• Keywords: Microfluidic chip

Nanohole array-based biosensors integrated with a microfluidic concentration gradient generator were used for imaging detection and quantification of ovarian cancer markers. Calibration curves based on controlled concentrations the analyte created using stepped diffusive mixing scheme. Quantification samples unknown was achieved by image-intensity comparison calibration curves. The first to detect immobilization marker antibodies, subsequently applied r-PAX8 (with limit about 5 nM dynamic range from 0.25 9.0 μg.mL−1). proposed biosensor demonstrated ability self-generating on-chip in an platform, representing further step towards development comprehensive lab-on-chip biomedical diagnostics nanohole array technology.