Highly sensitive fluorescent protein FRET detection using optofluidic lasers

• DOI: 10.1039/c3lc50207d
• Publication Date: 2013-03-13T14:10:21Z
• Authors: Qiushu Chen; Xingwang Zhang; Yuze Sun; Michael Ritt; Sivaraj Sivaramak...; Xudong Fan
• Keywords: Fluorescent protein; Yellow fluorescent protein; Acceptor; Protein Engineering

We achieved optofluidic protein lasing using genetically encoded fluorescent FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when and acceptor were brought close proximity, as compared only 17% conventional detection. Our work opens a door broad range of applications studying protein-protein interactions protein-drug interactions.