Membrane potential mediates the cellular binding of nanoparticles

• DOI: 10.1039/c3nr01667f
• Publication Date: 2013-05-09T15:12:36Z
• Authors: Edwin H. Shin; Ye Li; Umesh Kumar; Hursh V. Sureka; Xianren Zhang; Christine K. Payne
• Keywords: Cell membrane; Dissipative particle dynamics

The use of nanoparticles for cellular therapeutic or sensing applications requires to bind, adhere, the cell surface. While nanoparticle parameters such as size, shape, charge, and composition are important factors in binding, itself must also be considered. All cells have an electrical potential across plasma membrane driven by ion gradient. Under standard conditions gradient will result a -10 -100 mV with net negative charge on cytosolic face. Using combination flow cytometry fluorescence microscopy experiments dissipative particle dynamics simulations, we found that decrease leads decreased binding anionic nanoparticles. is general phenomenon, independent depolarization method, composition, type. Increased reverses this trend resulting increased cationic minimally affected due interaction surface proteins. influence especially when considering treatment detection diseases, cancer, which decreased.