Temperature-induced fluorescence enhancement of GFP chromophore containing copolymers for detection of Bacillus thermophilus

• DOI: 10.1039/c3py01559a
• Publication Date: 2013-12-16T16:57:43Z
• Authors: Yanjie Zheng; Guolin Li; Hongping Deng; Yue Su; Jianhua Liu; Xinyuan Zhu
• Keywords: Chromophore; Atom-transfer radical-polymerization; Side chain

Green fluorescent protein (GFP) chromophore based copolymers with temperature-induced emission enhancement properties were successfully prepared and applied for biodetection. First, diblock (PEG-b-PNIPAM-c) different poly(N-isopropylacrylamide) (PNIPAM) chain lengths synthesized by atom transfer radical polymerization (ATRP) employing a poly(ethylene glycol) (PEG) macroinitiator then modified GFP at one end through click reaction. Owing to the PNIPAM lengths, block exhibited thermoresponsive phase transitions adjustable lower critical solution temperature (LCST). Above LCST, fluorescence intensity of PEG-b-PNIPAM-c was enhanced dramatically, which could be attributed chromophore's conformational restriction collapse blocks. Moreover, increased length. Correspondingly, temperature-dependent in highly sensitive detection Bacillus thermophilus 102 cfu per mL limit.