Synthesis and characterization of a novel poly(vinyl alcohol) 3D platform for the evaluation of hepatocytes' response to drug administration

• DOI: 10.1039/c3tb20432d
• Publication Date: 2013-04-19T10:34:29Z
• Authors: Alessandra Stampella; Alessio Papi; Giuseppe Rizzitelli; Marco Costantini; Cristina Colosi; Andrea Barbetta; Mara Massimi; Laura Conti Devir...; Mariella Dentini
• Keywords: Vinyl alcohol; Characterization

Many whole cell-based assays in use today rely on flat, two-dimensional (2D) glass or plastic substrates that may not produce results characteristic of vivo conditions. In this study, a three-dimensional (3D) assay scaffold was fabricated using gas-in-foam templating technique. The made poly(vinyl alcohol), water-soluble synthetic polymer with excellent film-forming, emulsifying, and biocompatible properties widely used the biomedical field. preliminary rheological studies solution PVA surfactant permitted us to disclose significant physical parameters influence morphology ensuing materials. scaffolds obtained were subjected detailed analysis by light microscopy, Scanning Electron Microscopy (SEM), computed X-ray microtomography (μCT), infrared spectroscopy, mechanical testing. Morphological investigations showed produced are characterised average void interconnect diameters lying range 200-300 30-150 μm, respectively, suitable for cell infiltration. Two different cross-linking procedures adopted order modulate scaffolds. One bi-epoxide (PEGDGE), other based glutaraldehyde (GA). efficiency terms density two resulted very properties. Furthermore, article it is demonstrated how foams can be processed into uniform, porous films integrated multi-well 2D culture plates create 3D analogue. PEGDGE cross-linked tested C3A cells, human hepatocyte line, representing an appropriate model liver toxicity studies. Proliferation cytotoxicity indicated good viability throughout time, which also confirmed SEM analysis. Typical hepatic functions such as albumin urea production induction Cyp3A4 enzyme activity following drug administration satisfactory, thus proving construct maintaining specific functions.