Rapid amplification/detection of nucleic acid targets utilizing a HDA/thin film biosensor

• DOI: 10.1039/c4an00418c
• Publication Date: 2014-05-22T10:02:09Z
• Authors: Robert Jenison; Heidi Jaeckel; Joshua Klonoski; David Latorra; Jacinta Wiens
• Keywords: DNA, Bacterial; Methicillin-Resistant Staphylococcus aureus; 0303 health sciences; Time Factors; DNA Helicases; Biosensing Techniques; Equipment Design; Staphylococcal Infections; 3. Good health; 03 medical and health sciences; Bacterial Proteins; Nucleic Acids; Humans; Penicillin-Binding Proteins; Nucleic Acid Amplification Techniques; DNA Primers

A non-instrumented molecular diagnostics approach is described which integrates HDA amplification and thin film biosensor detection to create a platform capable of detecting pathogenic bacteria with high sensitivity and specificity.