An electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis, based on signal amplification of graphene and a gold nanoparticle–polyaniline nanocomposite

• DOI: 10.1039/c4an00976b
• Publication Date: 2014-07-31T09:32:12Z
• Authors: Chang Liu; Dongneng Jiang; Guiming Xiang; Linlin Liu; Fei Liu; Xiaoyun Pu
• Keywords: DNA, Bacterial; Aniline Compounds; Metal Nanoparticles; Biosensing Techniques; Electrochemical Techniques; Mycobacterium tuberculosis; Polymerase Chain Reaction; 01 natural sciences; 0104 chemical sciences; 3. Good health; Microscopy, Electron, Scanning; Graphite; Spectrophotometry, Ultraviolet; Gold

Due to its low growth rate and fastidious nature, Mycobacterium tuberculosis is difficult identify. Its rapid sensitive detection is, however, critical for the control of tuberculosis. Molecular biology, more recently electrochemical technology, have been exploited this pathogen. In present study, a novel DNA biosensor was developed highly specific insertion sequence IS6110 M. tuberculosis, using reduced graphene oxide–gold nanoparticles (rGO–AuNPs) as sensing platform gold nanoparticles–polyaniline (Au–PANI) tracer label amplification. Reduced oxide, which has large surface area, provided biocompatible matrix. Gold were electrodeposited on rGO modified electrode, not only increased immobilisation capture probe but also promoted electronic transfer. The Au–PANI nanocomposite exhibited good biocompatibility excellent activity. It therefore used detection, simple preparation process signal-on biosensor. With electroactivity nanocomposite, resulting high sensitivity over broad linear range, between 1.0 × 10−15 10−9 showed stability specificity provides new strategy clinical diagnostics probably pathogenic bacteria in general.