Comparison of different calibration approaches for chloramphenicol quantification in chicken muscle by ultra-high pressure liquid chromatography tandem mass spectrometry

• DOI: 10.1039/c4an01560f
• Publication Date: 2014-10-23T20:21:53Z
• Authors: Xiao-Dong Pan; Wei Jiang; Ping-Gu Wu
• Keywords: Tandem

Matrix-dependent signal suppression often occurs in quantitative analysis by ultra-high pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS). In this study, we investigated three calibration methods for compensation of on chloramphenicol (CAP) quantification chicken muscle. The data showed that the spiking recoveries solvent standard with a stable isotope labelled internal (SIL-IS) and matrix-matched SIL-IS were significantly higher than external (P < 0.05). When was used, standards prepared mobile phase no significant difference as those matrix > limit detection (LOD) matched 0.1 μg kg(-1), (including dissolved standard) 0.03 kg(-1).