Direct enzyme–substrate affinity determination by real-time hyperpolarized13C-MRS

• DOI: 10.1039/c4cc05418k
• Publication Date: 2014-09-11T13:00:37Z
• Authors: L. J. Friesen-Wal...; C. N. Wiens; T. P. Wade; K. Thind; K. P. Sinclair; Y. Hovav; J. M. Gomori; J. Sosna; C. A. McKenzie; R. Katz-Brull
• Keywords: Alcohol Oxidoreductases; 0303 health sciences; 03 medical and health sciences; Magnetic Resonance Spectroscopy; Choline

A direct determination ofKm, a fundamental measure of enzyme–substrate affinity, is now possible without sample manipulation by real-time hyperpolarized13C-MRS.