Cellular activity of siRNA oligonucleotides containing synthetic isomorphic nucleoside surrogates

• DOI: 10.1039/c4cc08809c
• Publication Date: 2014-12-05T14:01:06Z
• Authors: Dongwon Shin; Peter Lönn; Steven F. Dowdy; Yitzhak Tor
• Keywords: Nucleobase; Oligonucleotide synthesis

Singly and multiply modified synthetic siRNA oligonucleotides, containing isomorphic surrogate nucleobases, show high interference potency in cell culture, suggesting the highly RNA alphabet, based on a thieno[3,4-d]-pyrimidine core, is tolerated well by cellular silencing machinery.