Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis

• DOI: 10.1039/c5ay00244c
• Publication Date: 2015-05-01T15:00:12Z
• Authors: Douglas J. Kelly; Sean C. Warren; Dominic Alibhai; Sunil Kumar; Yuriy Alexandrov; Ian Munro; Anca Margineanu; Jessica McCormack; Natalie J. Welsh; Remigiusz A. Serwa; Emmanuelle Thinon; Mesayamas Kongsema; James McGinty; Clifford Talbot; Edward J. Murray; Frank Stuhmeier; Mark A. A. Neil; Edward W. Tate; Vania M. M. Braga; Eric W.-F. Lam; Christopher Dunsby; Paul M. W. French
• Keywords: Fluorescence-lifetime imaging microscopy

An HCA-FLIM instrument is presented alongside exemplar oligomerisation, intermolecular and intramolecular FRET assays that require robust measurement of small lifetime changes.