PRiME pass-through cleanup for the fast determination of aflatoxins in human serum by using LC-MS/MS

• DOI: 10.1039/c5ay03099d
• Publication Date: 2016-01-19T09:36:44Z
• Authors: Zhi-Yong Chen; Sheng Ying; Jin-Hong Liu; Ping-Ping Zhan; Yong-Gang Zhao
• Keywords: 01 natural sciences; 0104 chemical sciences; 3. Good health

A novel, simple and sensitive method based on the use of a PRiME (process, robustness, improvements, matrix effects, ease use) pass-through cleanup procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for determination 4 AFs, i.e., AFB1, AFB2, AFG1 AFG2 in human serum, was developed validated. The effective using Oasis HLB cartridge optimized extraction serum. obtained results demonstrated higher capacity recoveries between 92.1% 102%. limits detection (LODs) were 0.048 μg L−1, 0.069 0.043 L−1 0.14 respectively. Validation linearity, specificity, accuracy precision, as well application to analysis AFs 10 liver cancer volunteers applicability clinical studies.